Iris versicolor is a flowering herbaceous perennial plant, growing 10–80 cm (4–31 in) high. It tends to form large clumps from thick, creeping rhizomes. The unwinged, erect stems generally have basal leaves that are more than 1 cm (1⁄2 in) wide. Leaves are folded on the midribs so that they form an overlapping flat fan. The well developed blue flower has 6 petals and sepals spread out nearly flat and have two forms. The longer sepals are hairless and have a greenish-yellow blotch at their base. The inferior ovary is bluntly angled. Flowers are usually light to deep blue (purple and violet are not uncommon) and bloom during May to July. Fruit is a 3-celled, bluntly angled capsule. The large seeds can be observed floating in fall.
The species has been implicated in several poisoning cases of humans and animals who consumed the rhizomes, which have been found to contain a glycoside, iridin. The sap can cause dermatitis in susceptible individuals.